Abstract Hypertrichosis and dental anomalies may occur alone or in combination in the spectrum of many syndromes. To identify genetic entities characterized by hypertrichosis and dental anomalies, a search was performed in the Mendelian Inheritance in Man database with the terms “hypertrichosis” or “hirsutism” and “tooth” or “dental abnormalities.” Nondependent androgen metabolism disturbances were classified as hypertrichosis. Genetic entities with hypertrichosis and dental anomalies were included in the study. Additional searches were performed in the PubMed and Orphanet databases, when necessary, in order to include data from scientific articles. An integrative analysis of the genes associated with the identified syndromes was conducted using STRING to characterize biological processes, pathways, and interactive networks. The p-values were subjected to the false discovery rate for the correction of multiple tests. Thirty-nine syndromes were identified, and dental agenesis was the most frequent dental anomaly present in 41.02% (n = 16) of the syndromes. Causative genes were identified in 33 out of 39 genetic syndromes. Among them, 39 genes were identified, and 38 were analyzed by STRING, which showed 148 biological processes and three pathways that were statistically significant. The most significant biological processes were the disassembly of the nucleosome (GO:0006337, p = 1.09e-06), chromosomal organization (GO:0051276, p = 1.09e-06) and remodeling of the chromatin (GO: 0006338, p = 7.86e-06), and the pathways were hepatocellular carcinoma (hsa05225, p = 5.77e-05), thermogenesis (hsa04714, p = 0.00019), and cell cycle (hsa04110, p = 0.0433). Our results showed that the identification of hypertrichosis and dental anomalies may raise the suspicion of one of the thirty-nine syndromes with both phenotypes.

Abstract: The aim of this study was to describe the oral health services offered by the Unified Health System in the northern macro-region of Minas Gerais, Brazil, during the COVID-19 pandemic. This was an observational, cross-sectional and descriptive study. Data collection took place between August and September 2020 through the completion of online questionnaires by oral health managers in the northern municipalities of Minas Gerais and by consultation of information available in the Special Epidemiological Bulletin Coronavirus North Macro-region n° 14. After collection, data were transferred to SPSS® (Statistical Package for the Social Sciences for Windows, version 24.0). Analyses were performed using frequency, mean, standard deviation and percentages. It was found that all included municipalities suspended elective treatments and maintained emergency dental care at all levels of care. In 62.5% of municipalities there were oral health professionals contaminated by COVID-19. Oral health teams implemented specific interventions to address the pandemic,with the most frequent being tele-orientation (74.7%), use of sanitary barriers (72%), and telemonitoring (68%). At the beginning of the pandemic, 62.5% of municipalities lacked personal protective equipment. Comsidering these results, it was found that the COVID-19 pandemic impacted the supply of dental services in northern Minas Gerais due to the suspension of elective care, contamination of professionals, lack of personal protective equipment, and development of new interventions. Thus, oral health teams had to adapt to new contexts of health interventionsto face COVID-19 and maintain dental care services.

Abstract We analyzed the distribution characteristics of the scientific production of Brazilian dentistry researchers of the Brazilian National Council for Scientific and Technological Development. The Lattes curriculum data of 211 researchers from Oral Pathology, Oral Surgery, and Oral Medicine fields were included (2018–2020). Over their academic career, 39 researchers published 7,555 papers (average, 175 articles/researcher); 3,876/7,555 papers were indexed in the Web of Science. During 2018–2020, 1,440/7,555 (19%) papers were published. Brazilian dental researchers contribute significantly to international research by participating in scientific journals’ editorial boards, evaluating research promotion agencies, training researchers, and contributing to scientific and technological development.

Abstract: Kawasaki disease (KD) is a vasculitis with predilection for coronary arteries. Due to a lack of reliable confirmatory laboratory tests, the diagnosis of KD is based on a characteristic pattern of clinical findings that appear in a typical temporal sequence. The diagnostic criteria have been periodically modified and the American Heart Association has proposed the most recent guidelines for its diagnosis. However, patients may have incomplete or atypical forms of KD and diagnosis can often be difficult. Because oropharyngeal manifestations are a common and important feature for diagnosing KD and recent studies have hypothesized a possible association between KD and the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), in this review we highlight the importance of dentists in the diagnosis of KD and its potential association with SARS-CoV-2.

O trabalho foi realizado com objetivo de avaliar a qualidade das sementes de quatro cultivares de soja (BRS 133, BRS 206, BRS 239 e CD 202), colhidas em quatro épocas (Estádio R7 e 7, 14 e 21 dias após a primeira época) e produzidas em dois locais do Estado do Mato Grosso do Sul (Sidrolândia e Dourados), nos anos agrícolas de 2004/05 e 2005/06. O delineamento experimental utilizado foi em blocos casualizados com três repetições e tratamentos arranjados no esquema de parcelas subdivididas. As parcelas foram constituídas pelas cultivares de soja e as subparcelas pelas épocas de colheita. A germinação foi avaliada pelo teste de germinação; teste de envelhecimento acelerado e o vigor pelo teste de frio sem solo, teste de emergência das plântulas em substrato de areia e teste de sanidade. Os dados coletados nos diferentes locais foram submetidos à análise de variância conjunta de parcelas subdivididas e, na presença de interação significativa, foram realizados os desdobramentos necessários para os dois anos agrícolas, separadamente. No ano agrícola 2004/05, as cultivares BRS 239 e CD 202 apresentaram-se com elevada porcentagem de germinação para a produção de sementes na região de Dourados. No ano agrícola 2005/06, a cultivar BRS 239 foi a que apresentou melhor germinação e vigor. A cultivar BRS 239 apresenta menor índice de infecção de microrganismos em relação a cultivar BRS 206. Melhor qualidade de sementes é obtida das sementes colhidas aos sete dias após o estádio R7. Em relação à qualidade sanitária das sementes, à medida que se retarda a época de colheita, a porcentagem total de microrganismos aumenta linearmente para todas as cultivares.

The object of this study was to evaluate the seed quality of four soybean cultivars (BRS 133, BRS 206, BRS 239 and CD 202), harvested at four different times (R7 stage and 7, 14 and 21 days after the first period), at two locations in Mato Grosso do Sul state, Brazil (Sidrolândia and Dourados), in the 2004/05 and 2005/06 crop seasons. The experimental design was a randomized block with three replications in a split-plot. The plots and subplots were composed of soybean cultivars and harvest periods, respectively. Germination was evaluated by the standard germination test and vigour by the cold test without soil, the test of seedling emergence in a sand substrate, accelerated aging test and also by the health test. The data collected at the different sites were submitted to an analysis of variance of the split plots and, in the presence of significant interaction, the data were separated for the two crop years. In the 2004/ 2005 crop season, the BRS 239 and CD 202 soybean varieties showed high percentage germination for the production of seeds in the Dourados area. The best seed quality was obtained by harvesting 7 days after the R7 stage. BRS 239 showed less infection by microorganisms compared to cultivar BRS. In the 2005/ 2006 crop season, the BRS 239 cultivar showed the best germination and vigour. In relation to seed health quality, with greater delays in harvesting, the total percentage of microorganisms increases linearly for all the cultivars.

O objetivo do presente trabalho foi avaliar a qualidade fisiológica e sanitária das sementes, em resposta à aplicação do biorregulador Stimulate® na cultura da soja. Para tanto, sementes de soja da cultivar BRS 246 RR foram semeadas no mês de outubro dos anos agrícolas de 2007/2008 e 2008/2009, no delineamento experimental em blocos completos com os tratamentos casualizados, com quatro repetições. Os tratamentos, arranjados em esquema fatorial, foram compostos pela combinação do tratamento de sementes com o biorregulador (sem e com 0,500 L 100 kg-1 de sementes) e cinco doses do produto (0; 0,125; 0,250; 0,375 e 0,500 L ha-1) aplicadas via foliar, em dois estádios de desenvolvimento da cultura (V5 ou R3). O uso do biorregulador Stimulate® altera positivamente qualidade das sementes, sobretudo quando as aplicações foliares ocorrem no estádio V5.

The objective of this study was to evaluate the physiological and sanitary quality of soybean seeds treated with the growth regulator Stimulate®. Seeds of the cultivar BRS 246 RR were planted in October of crop years 2007/2008 and 2008/2009, in a randomized complete block design with four replications. The treatments were set out in a factorial, with a combination of seed treatment and the growth regulator (with and without 0.500 L.100 kg-1 bean) at five product dosages (0, 0.125, 0.250, 0.375 and 0.500 L ha-1) with foliar application at two developmental crop stages (V5 or R3). The application of Stimulate® positively altered seed quality, especially at the V5 stage.

O cultivo de soja em safrinha é prática que se consolida, em termos de opção, para a entressafra, podendo servir também à produção de sementes de soja. O presente trabalho foi conduzido com objetivo de avaliar o efeito de diferentes épocas de semeadura, na safrinha da soja, sobre a qualidade fisiológica e sanitária das sementes de três cultivares de soja, na região oeste do Estado do Paraná. Para tanto, foram conduzidos quatro ensaios, no ano de 2005, com delineamento em blocos completos casualizados, instalando-se um ensaio em quatro épocas de semeadura (15/01, 30/01, 15/02 e 15/03). Os cultivares estudados foram CD 202, CD 215 e CD 216. As sementes foram avaliadas por meio dos testes de germinação e de vigor (primeira contagem e envelhecimento acelerado) e a sanidade por meio do "blotter test". As sementes produzidas no período de safrinha no ano de 2005, para as condições edafoclimáticas da região oeste do Estado do Paraná, não foram favoráveis à obtenção de sementes de alta qualidade fisiológica.

Soybean seeds produced in out season in west of Paraná state. The soybean culture in out season as a practice that consolidates in terms of an option between harvests, could also serve to the production of soybean seeds. The present work was carried out with the objective of evaluating the effect of sowing in out season, on physiological and sanitary quality of seeds of three soybeans cultivars, in west of Paraná state. For such way were conducted four assays in the year of 2005, arranged in a completely randomized block design, with each assay sown in different dates (01/15, 01/30, 02/15 and 03/15). The studied cultivars were CD 202, CD 215 and CD 216. The seeds were evaluated by germination and vigor tests (first counting and accelerated aging) and of health. The seeds produced in "out season" in the year of 2005, to the environmental conditions of the west of Paraná state were not favorable to the attainment of high physiological quality seeds.

A semeadura antecipada da soja, particularmente utilizando cultivares precoces, é prática que vem sendo adotada por muitos produtores paranaenses com o intuito de antecipar a colheita e viabilizar o cultivo de milho safrinha. Visando avaliar a influência da antecipação da semeadura na composição química e na produtividade da soja, foram instalados ensaios de competição de cultivares na unidade da COODETEC, em Palotina (PR). O delineamento foi em blocos casualizados, desenvolvido em dois anos agrícola (2003/2004 e 2004/2005), com três cultivares precoces (CD 202, CD 215 e CD 216), semeadas em cinco épocas (15/9, 30/9, 15/10, 30/10 e 15/11). Foram determinados os teores de óleo e proteínas nos grãos. Partindo-se do rendimento de grãos nas parcelas, foram calculadas as produtividades em kg ha-1. Os dados coletados foram submetidos à análise de variância conjunta e realizados os desdobramentos das interações quando necessários. Os resultados permitiram concluir que a antecipação da semeadura influenciou na produtividade e composição química dos grãos.

Early soybean sowing, especially using early cultivars is a practice that is adopted by many growers from the State of Paraná in order to anticipate harvest and to enhance growing corn crop during the fall. Experiments of soybean cultivars competition trials were carried out at COODETEC in the city of Palotina, Paraná State, aiming to evaluate the influence of early sowing on the chemical composition of seeds, as well as yield. A randomized block design, comprised by two crop years (2003/2004 and 2004/2005), three early cultivars (CD 202, CD 215 and CD 216); and five sowing times (09/15, 09/30, 10/15, 10/30 and 11/15) was utilized. Also, oil and protein content were also determined. The productivity (kg ha-1) was calculated using the yield of grain in each parcel. All data were submitted to variance analysis of group experiments along with all interactions and necessary adjustments. Results allowed to conclude that early sowing can influence grain yield and seed chemical composition.

O objetivo do trabalho foi avaliar o efeito da aplicação de biorregulador no desempenho agronômico das plantas e na produtividade de sementes da cultura da soja cultivar CD 216. O trabalho foi desenvolvido em dois anos agrícolas (2005/06 e 2006/07). Os tratamentos foram compostos pela combinação do tratamento de sementes (0 e 500 mL 100 kg-1 de sementes) com três doses de biorregulador (250; 375 e 500 mL ha-1) aplicadas via foliar em dois estádios de desenvolvimento da cultura (V5 e R3), mais a testemunha sem aplicação. As características avaliadas foram a altura das plantas, número de vagens por planta, produtividade e massa de 1.000 sementes. As características altura das plantas e número de vagens por planta não foram influenciadas pela aplicação do biorregulador nos dois anos agrícolas. No ano agrícola de 2005/06, a maior produtividade de sementes (2.927 kg ha-1) foi alcançada pela aplicação da dose de 211 mL ha-1 de biorregulador via foliar no estádio R3, associado ao tratamento de sementes. No segundo ano agrícola, não houve diferença significativa (p > 0,05) entre os tratamentos na produtividade de sementes, muito embora os resultados alcançados tenham sido superiores aos obtidos no primeiro ano. Um dos princípios básicos para melhorar a eficácia do biorregulador na cultura da soja é a condição climática adversa.

The purpose of the work was to verify the effect of the application of a plant growth regulator on the agronomic traits of plants and on soybean seed yield, cultivar CD 216. The work was developed during two agricultural years (2005/06 and 2006/07). The treatments were composed by the combination of seed treatments (0 and 500 mL 100 kg-1 of seeds) with three doses of plant growth regulator (250, 375 and 500 mL ha-1) applied to the leaves in two stages of the culture's development (V5 and R3), plus the control without application. The features evaluated were: plant height, number of seedpods per plant, yield and mass of one thousand seeds. The features of plant height and number of seedpods per plant were not influenced by the application of the plant growth regulator in the two agricultural years. In the agricultural years of 2005/06, the greater seed yield (2.927 kg ha-1) was reached with the application of 211 mL ha-1 of plant growth regulator to the leaves at the R3 stage, together with seed treatment. In the second agricultural year there was no significant difference (p > 0.05) between the treatments in seed yield, although the results were superior to those obtained in the first year. One of the basic principles to improve the efficacy of the plant growth regulator in soybean crops is an adverse climatic condition.

OBJETIVO: Analisar fatores que influenciam a ocorrência de metástase linfática cervical e a sobrevida nos tumores malignos epiteliais da glândula parótida. MÉTODO: Analisamos retrospectivamente os prontuários de 150 pacientes tratados em nossa instituição de 1974 a 1998. Vinte e quatro pacientes foram excluídos do estudo por não terem sido tratados primariamente por cirurgia. O 126 pacientes restantes foram submetidos a parotidectomia e incluídos neste estudo. Setenta e quatro pacientes tiveram sua cirurgia complementada por radioterapia pós-operatória. Trinta e quatro pacientes foram submetidos ao esvaziamento cervical associado a parotidectomia. A idade média foi de 49 anos. Todos os pacientes foram estadiados pela Classificação TNM da UICC de 1997, sendo 49 pacientes estágio I, 27 estágio II, 22 estágio III, and 28 estágio IV. A influência dos fatores analisados na ocorrência de metástase cervical foi estabelecida pelo teste do chi quadrado e por análise multivariada. A influência de fatores prognósticos na sobrevida específica de doença (SED) em 5 e 10 anos foi estabelecida pelo método de Kaplan-Meier e pelo teste log-rank. RESULTADOS: O tipo histopatológico de 40 pacientes foi o carcinoma mucoepidermóide, de 18 pacientes o adenocarcinoma (SOE), de 18 pacientes o carcinoma de células acinares, de 15 pacientes o carcinoma adenóide cístico, de 11 pacientes o carcinoma exadenoma pleomórfico, de 11 pacientes o carcinoma de ducto salivares, e de 13 pacientes outras histopatologias. Vinte e cinco pacientes apresentaram recidivas, 17 recidivas locais, quatro recidivas regionais, e quatro recidivas loco-regionais. A incidência geral de metástase linfática cervical foi de 17,5%. Metástases linfáticas cervicais ocultas ocorreram em cinco pacientes daqueles submetidos a esvaziamento cervical profilático. A presença de paralisia facial no diagnóstico, a idade, o estágio T, e o grau de malignidade foram relacionados a ocorrência de metástase linfática cervical na análise multivariada. Cinco fatores influenciaram negativamente o prognóstico, estágio T3-T4, alto grau de malignidade, presença de metástase cervical, paralisia facial no diagnóstico, e idade acima de 50 anos. A SED em 10 anos foi de 97% para o estágio I, 81% para o estágio II, 56% para o estágio III, e 20% para estágio IV. CONCLUSÕES: O grau de malignidade e o estágio foram os fatores prognósticos mais importantes. Apesar de ser um estudo retrospectivo e não randomizado, nossos dados sugerem que o esvaziamento cervical profilático deve ser considerado em casos de tumores de alto grau de malignidade, estágio T3-T4, e na presença de paralisia facial no diagnóstico.

BACKGROUND: Factors influencing the occurrence of neck metastasis and survival in patients with parotid malignant epithelial tumors are analyzed. METHODS: One hundred fifty patients treated at our institution from 1974 to 1998 were retrospectively reviewed. Twenty four patients were not treated by surgery and were excluded from this study. The remaining 126 patients were operated on and 74 patients had postoperative radiotherapy. Thirty four patients were treated with parotidectomy plus neck dissection. The mean age was 49 years old. According to the UICC/1997 TNM Classification 49 patients were Stage I, 27 were Stage II, 22 were Stage III, and 28 were Stage IV. The influence of selected factors on 10 years disease-specific survival was analyzed using Kaplan-Meier actuarial method and log-rank test. RESULTS: Forty patients had mucoepidermoid carcinoma, 18 patients adenocarcinoma, 18 patients acinic cell carcinoma, 15 patients adenoid cystic carcinoma, 11 patients carcinoma ex pleomorphic adenoma, 11 patients salivary duct carcinoma, and 13 patients other pathology. Recurrences occurred on 27 patients, 17 local, 4 had neck recurrences, and 4 loco-regional recurrences. Overall incidence of neck metastasis was 17.5%. Occult neck metastasis occurred in 5 patients who underwent elective neck dissection. Facial nerve dysfunction, age, T stage, grade, and histology were related to the recurrence of neck metastasis in univariate analysis. Prognoses were negatively influenced by five factors: T3- T4 stage, high malignancy grade, presence of cervical metastases, facial nerve palsy at first presentation and age higher then 50 years old. Ten years disease-specific survival was 97% for stage I, 81% for stage II, 56% for stage II, and 20% for stage IV. CONCLUSION: Tumor grade and stage were the most important prognostic factors. In spite of lack of prospective randomized published studies, recommendations to do elective neck dissections in high grade tumors, T3- T4 stage tumors, and facial nerve palsy at presentation should be considered.

OBJETIVO: Analisar fatores prognósticos na sobrevida livre de doença e específica dos pacientes com carcinomas de células de Hürthle. MÉTODO: Estudo retrospectivo de 28 pacientes tratados na Seção de Cirurgia de Cabeça e Pescoço do HCI - INCa, objetivando coletar dados demográficos, clínicos e histopatológicos e relacioná-los com a sobrevida livre de doença e sobrevida global específica. O estadiamento foi realizado conforme a AJCC de 2002. Para análise estatística foi utilizado o programa Epi info 2002, considerando-se significativo estatisticamente o valor de p < 0,05. A análise da curva de sobrevida foi realizada utilizando-se o método de Kaplan-Meyer. O tempo médio de seguimento foi de 69,3 meses (10 - 230 meses). RESULTADOS: Foram avaliados 28 pacientes, com idade média de 50,8 anos. As variáveis que apresentaram impacto prognóstico quanto a recidiva foram o estágio mais avançado da doença (p = 0,03), a presença de metástase a distância (p = 0,03 e principalmente o padrão histológico de invasão maciça (p = 0,0027). Quando relacionado as variáveis estudadas com a mortalidade, o padrão de invasão histológica maciça (p = 0,02), o maior tamanho do tumor (p = 0,013) e principalmente a presença de metástases a distância (p = 0,0056) apresentaram relação com significância estatística. A sobrevida livre de doença foi de 72% e 55% e a sobrevida global de 87% e 77% em 5 e 10 anos respectivamente. CONCLUSÃO: A presença de metástase à distância e o padrão histológico de invasão maciça apresentaram relação estatisticamente significativa com a sobrevida livre de doença e específica.

OBJECTIVE: Analyze prognostic factors for disease free survival and specific survival in patients with Hurthle Cell Carcinoma. METHODS: We analyzed, retrospectively, the files of 28 patients treated in the Service of Head and Neck Surgery of the Brazilian National Cancer Institute/INCA from January 1983 to December 2002. Data regarding demographics, clinical aspects, therapeutics and histopathology were collected and related to disease-free survival and disease-specific survival. Patients were classified according to the AJCC 2002 staging system. Data was analyzed by the Epi Info 2002 software. Survival was analyzed by the Kaplan-Meier actuarial method. The median follow-up time was 69 months (range from 10 to 230). RESULTS: Advanced stages (p=.03), distant metastases (p=.03) and, particularly, the pattern of capsular invasion (widely invasive) (p=.0027) influenced negatively the prognosis in the disease-free survival. Analyzing the relationship between specific survival, the pattern of capsular invasion (widely invasive) (p=.02), T stage (p=.013) and, particularly, the presence of distant metastases (p=.0056) presented statistical significance. Five and 10 year disease-free survival was 72% and 55%, respectively, and 5 and 10 year disease-specific survival was 87% and 77%, respectively. CONCLUSION: The presence of distant metastases and widely invasive tumors were the most important negative prognostic factors associated with Hurthle Cell Carcinomas in our study.

Os sistemas de preparo e manejo do solo determinam as condições físicas para o crescimento das plantas e produtividade das culturas. O objetivo deste estudo é o de avaliar algumas propriedades físicas de um Latossolo Vermelho distrófico, cultivado com mandioca, após dois anos de implantação em diferentes sistemas de preparo do solo. Os tratamentos utilizados foram: Plantio Direto (mobilização do solo somente ao longo das linhas de plantio); Preparo Mínimo (escarificação a 0,30 m, seguido de gradagem niveladora) e Preparo Convencional (aração, com arado de aiveca a uma profundidade de 0,25 m, seguido de gradagem niveladora). Avaliaram-se, nas camadas de 0-0,10 m e 0,10-0,20 m, as seguintes propriedades físicas do solo: densidade, volume de macroporos, microporos, a porosidade e a resistência do solo à penetração das raízes, a cada 0,05 m, até à profundidade de 0,40 m, em três diferentes épocas. Constataram-se maiores valores de densidade do solo e menores valores de macroporosidade na camada de 0-0,10 m no plantio direto e preparo mínimo do solo. Resultados similares foram obtidos na avaliação da resistência do solo à penetração, independente da época de avaliação e da umidade do solo. Os sistemas de preparo mínimo e preparo convencional do solo proporcionam condições físicas menos restritivas ao crescimento das plantas, quando comparado com o plantio direto. Avaliações de médio-longo prazo são necessárias face às mudanças dinâmicas na qualidade física do solo, impostas pelos sistemas de preparo e manejo do solo e da cultura.

Soil tillage and management affect soil physical conditions for plant growth and crop productivity. The objective of this research was to evaluate some soil physical properties of an Typic Haplorthox located in the Northwest region of the State of Paraná, Brazil, after two years of different tillage systems, with same crop. The tillage systems used were: No-tillage (cultivated only along the crop row - NT), Minimum Tillage (subsoiling to the 0.30 m depth, followed by leveling - MT) and Conventional Tillage (plowing with moldboard plow to the 0.25 m depth, followed by leveling - CT). The following soil physical properties were evaluated at the depths of 0-0.10 m and 0.10-0.20 m: soil bulk density (Bd), soil porosity, and the soil resistance to root penetration (SRP), which was evaluated at each 0.05 m up to 0.4m, during three different periods. The results suggest showed higher values of Bd and smaller values of macroporosity in the 0-0,10 m layer for NT and MT. Similar results were obtained for SRP, independently of the time of evaluation and soil water content. MT and CT may provide favorable soil physical conditions to the growth of the crop when compared to NT. Medium to long-term evaluations are necessary due to the dynamic changes in soil physical quality, result of the tillage system, soil and crop management.